A SYBR inexperienced I real-time polymerase chain response

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hydropsfetalis

A SYBR inexperienced I real-time polymerase chain response (PCR) assay for detection and quantification of Trichomonas gallinae

Trichomonas gallinae are parasitic flagellates of significance in wild and residential birds. The parasite is worldwide distributed, and Columbine birds are its foremost host. Current evaluation focuses completely on epidemiological and phylogenetic analysis. Nonetheless, there could also be nonetheless a lack of expertise regarding parasite-host interaction or treatment progress.

Precise-time PCR is a helpful gizmo for diagnostic and quantification of gene copies in a determined sample. By amplification of a 113-bp space of the 18S small subunit ribosomal RNA gene, a SYBR green-based real-time PCR assay was developed.

A daily curve was prepared for quantification analysis. Assay effectivity, linearity, and dissociation analysis have been effectively carried out. Specificity, sensibility, and reproducibility analysis have been examined. This assay is perhaps a helpful gizmo not only for diagnostic capabilities however moreover for future in vivo and in vitro T. gallinae analysis.

hydropsfetalis
hydropsfetalis

GPR12 Antibody

44910-100ul 100ul
EUR 252

GPR12 Antibody

44910-50ul 50ul
EUR 187

GPR12 Antibody

DF2721 200ul
EUR 304
Description: GPR12 antibody detects endogenous levels of total GPR12.

GPR12 antibody

70R-49804 100 ul
EUR 244
Description: Purified Polyclonal GPR12 antibody

GPR12 Antibody

1-CSB-PA008905
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/5000

GPR12 Antibody

1-CSB-PA009742LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

GPR12 Antibody

ABD2721 100 ug
EUR 438

GPR12

RA30050 50 ug
EUR 618

Gpr12/ Rat Gpr12 ELISA Kit

ELI-09764r 96 Tests
EUR 886

GPR12 Conjugated Antibody

C44910 100ul
EUR 397

GPR12 Polyclonal Antibody

ABP54586-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90
  • Applications tips:
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

GPR12 Polyclonal Antibody

ABP54586-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90
  • Applications tips:
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

GPR12 Polyclonal Antibody

ABP54586-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90
  • Applications tips:
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

GPR12 Polyclonal Antibody

ES5585-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA

GPR12 Polyclonal Antibody

ES5585-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA

GPR12 siRNA

20-abx902268
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

GPR12 siRNA

20-abx918427
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

GPR12 siRNA

20-abx918428
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

GPR12 Antibody, HRP conjugated

1-CSB-PA009742LB01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

GPR12 Antibody, FITC conjugated

1-CSB-PA009742LC01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

GPR12 Antibody, Biotin conjugated

1-CSB-PA009742LD01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

GPR12 Blocking Peptide

DF2721-BP 1mg
EUR 195

GPR12 Blocking Peptide

20-abx062679
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
  • Shipped within 5-10 working days.

Polyclonal GPR12 Antibody (Extracellular Domain)

APR16480G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (Extracellular Domain). This antibody is tested and proven to work in the following applications:

Polyclonal GPR12 Antibody (N-Terminus)

APR16481G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (N-Terminus). This antibody is tested and proven to work in the following applications:

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

GPR12 ELISA KIT|Human

EF005024 96 Tests
EUR 689

Rat GPR12 shRNA Plasmid

20-abx986567
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

Human GPR12 shRNA Plasmid

20-abx951900
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

Mouse GPR12 shRNA Plasmid

20-abx970621
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

GPR12 Recombinant Protein (Human)

RP061035 100 ug Ask for price

GPR12 Recombinant Protein (Rat)

RP203285 100 ug Ask for price

GPR12 Recombinant Protein (Rat)

RP203288 100 ug Ask for price

GPR12 Recombinant Protein (Mouse)

RP139421 100 ug Ask for price

GPR12 Recombinant Protein (Mouse)

RP139424 100 ug Ask for price

Polyclonal GPR12 Antibody - C-terminal region

APR16482G 0.05mg
EUR 528
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 - C-terminal region. This antibody is tested and proven to work in the following applications:

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx009208
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx147559
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx324375
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx338465
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Gpr12 ORF Vector (Rat) (pORF)

ORF067763 1.0 ug DNA
EUR 506

Gpr12 ORF Vector (Rat) (pORF)

ORF067764 1.0 ug DNA
EUR 506

GPR12 ORF Vector (Human) (pORF)

ORF020346 1.0 ug DNA
EUR 405

Gpr12 ORF Vector (Mouse) (pORF)

ORF046475 1.0 ug DNA
EUR 506

Gpr12 ORF Vector (Mouse) (pORF)

ORF046476 1.0 ug DNA
EUR 506

G Protein Coupled Receptor 12 (GPR12) Antibody (HRP)

20-abx335850
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody (FITC)

20-abx335851
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody (Biotin)

20-abx335852
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Gpr12 sgRNA CRISPR Lentivector set (Rat)

K7010601 3 x 1.0 ug
EUR 339

Gpr12 sgRNA CRISPR Lentivector set (Mouse)

K3382801 3 x 1.0 ug
EUR 339

GPR12 sgRNA CRISPR Lentivector set (Human)

K0889201 3 x 1.0 ug
EUR 339

Gpr12 sgRNA CRISPR Lentivector (Rat) (Target 1)

K7010602 1.0 ug DNA
EUR 154

Gpr12 sgRNA CRISPR Lentivector (Rat) (Target 2)

K7010603 1.0 ug DNA
EUR 154

Gpr12 sgRNA CRISPR Lentivector (Rat) (Target 3)

K7010604 1.0 ug DNA
EUR 154

Gpr12 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3382802 1.0 ug DNA
EUR 154

Gpr12 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3382803 1.0 ug DNA
EUR 154

Gpr12 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3382804 1.0 ug DNA
EUR 154

GPR12 sgRNA CRISPR Lentivector (Human) (Target 1)

K0889202 1.0 ug DNA
EUR 154

GPR12 sgRNA CRISPR Lentivector (Human) (Target 2)

K0889203 1.0 ug DNA
EUR 154

GPR12 sgRNA CRISPR Lentivector (Human) (Target 3)

K0889204 1.0 ug DNA
EUR 154

GPR12 Protein Vector (Rat) (pPB-C-His)

PV271050 500 ng
EUR 603

GPR12 Protein Vector (Rat) (pPB-N-His)

PV271051 500 ng
EUR 603

GPR12 Protein Vector (Rat) (pPM-C-HA)

PV271052 500 ng
EUR 603

GPR12 Protein Vector (Rat) (pPM-C-His)

PV271053 500 ng
EUR 603

GPR12 Protein Vector (Rat) (pPB-C-His)

PV271054 500 ng
EUR 603

GPR12 Protein Vector (Rat) (pPB-N-His)

PV271055 500 ng
EUR 603

GPR12 Protein Vector (Rat) (pPM-C-HA)

PV271056 500 ng
EUR 603

GPR12 Protein Vector (Rat) (pPM-C-His)

PV271057 500 ng
EUR 603

GPR12 Protein Vector (Mouse) (pPB-C-His)

PV185898 500 ng
EUR 603

GPR12 Protein Vector (Mouse) (pPB-N-His)

PV185899 500 ng
EUR 603

GPR12 Protein Vector (Mouse) (pPM-C-HA)

PV185900 500 ng
EUR 603

GPR12 Protein Vector (Mouse) (pPM-C-His)

PV185901 500 ng
EUR 603

GPR12 Protein Vector (Mouse) (pPB-C-His)

PV185902 500 ng
EUR 603

GPR12 Protein Vector (Mouse) (pPB-N-His)

PV185903 500 ng
EUR 603

GPR12 Protein Vector (Mouse) (pPM-C-HA)

PV185904 500 ng
EUR 603

GPR12 Protein Vector (Mouse) (pPM-C-His)

PV185905 500 ng
EUR 603

GPR12 Protein Vector (Human) (pPB-C-His)

PV081381 500 ng
EUR 552

GPR12 Protein Vector (Human) (pPB-N-His)

PV081382 500 ng
EUR 552

GPR12 Protein Vector (Human) (pPM-C-HA)

PV081383 500 ng
EUR 552

GPR12 Protein Vector (Human) (pPM-C-His)

PV081384 500 ng
EUR 552

Gpr12 3'UTR Luciferase Stable Cell Line

TU108969 1.0 ml Ask for price

Gpr12 3'UTR Luciferase Stable Cell Line

TU205326 1.0 ml Ask for price

Gpr12 3'UTR GFP Stable Cell Line

TU158969 1.0 ml Ask for price

Gpr12 3'UTR GFP Stable Cell Line

TU255326 1.0 ml Ask for price

GPR12 3'UTR GFP Stable Cell Line

TU059144 1.0 ml
EUR 1394

GPR12 3'UTR Luciferase Stable Cell Line

TU009144 1.0 ml
EUR 1394

GPR12 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV626605 1.0 ug DNA
EUR 682

GPR12 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV626609 1.0 ug DNA
EUR 682

GPR12 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV626610 1.0 ug DNA
EUR 682

GPR12 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV652531 1.0 ug DNA
EUR 682

GPR12 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV652535 1.0 ug DNA
EUR 682

GPR12 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV652536 1.0 ug DNA
EUR 682

Mouse G- protein coupled receptor 12, Gpr12 ELISA KIT

ELI-09763m 96 Tests
EUR 865

Human G- protein coupled receptor 12, GPR12 ELISA KIT

ELI-19453h 96 Tests
EUR 824

Rat G Protein Coupled Receptor 12 (GPR12) ELISA Kit

abx391367-96tests 96 tests
EUR 911
  • Shipped within 5-12 working days.

Human G Protein Coupled Receptor 12 (GPR12) ELISA Kit

abx384941-96tests 96 tests
EUR 911
  • Shipped within 5-12 working days.

Mouse G Protein Coupled Receptor 12 (GPR12) ELISA Kit

abx389367-96tests 96 tests
EUR 911
  • Shipped within 5-12 working days.

Gpr12 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K7010605 3 x 1.0 ug
EUR 376

Gpr12 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K3382805 3 x 1.0 ug
EUR 376

GPR12 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K0889205 3 x 1.0 ug
EUR 376

Gpr12 ELISA Kit| Rat G-protein coupled receptor 12 ELISA Kit

EF018723 96 Tests
EUR 689

Gpr12 ELISA Kit| Mouse G-protein coupled receptor 12 ELISA Kit

EF015000 96 Tests
EUR 689

GPR12 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV626606 1.0 ug DNA
EUR 682

GPR12 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV626607 1.0 ug DNA
EUR 740

GPR12 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV626608 1.0 ug DNA
EUR 740

GPR12 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV652532 1.0 ug DNA
EUR 682

GPR12 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV652533 1.0 ug DNA
EUR 740

GPR12 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV652534 1.0 ug DNA
EUR 740

Enchancment of a typical RT-PCR assay for grapevine vitiviruses

The genus Vitivirus inside the family Betaflexiviridae comprises eleven viruses acknowledged to infect grapevine: grapevine vitiviruses A, B, D, E, F, G, H, I, J, L and M (GVA-GVM). Three of these viruses, GVA, GVB and GVD, have been associated to the etiology of rugose wood sickness in grapevine and set off agronomically important losses. The alternative vitiviruses have been additional simply recently discovered and their outcomes on grapevine are undetermined. To certify grape supplies for propagation as virus examined, an updated reverse transcription PCR (RT-PCR) assay to detect all acknowledged vitiviruses is fascinating.

To carry out this, various grapevine vitivirus sequences have been aligned on the amino acid diploma to hunt for conserved motifs. Two extraordinarily conserved motifs have been found at a superb distance for RT-PCR detection inside the RNA-dependent RNA polymerase space of the replicase protein. The amino acid motifs have been once more translated to create degenerate primers and used to effectively amplify all eleven grapevine vitiviruses. The RT-PCR primers have been used to examine a panel of vitivirus-infected vines for inclusivity along with vines contaminated with fastidiously related viruses inside the Betaflexiviridae family (i.e. grapevi)

ne pinot gris virus and grapevine rupestris stem pitting-associated virus) for exclusivity. Broader use of these primers to detect vitiviruses in numerous plant hosts was investigated. In summary, an end-point RT-PCR assay that detects all the acknowledged grapevine vitiviruses and doubtless totally different members of the genus Vitivirus has been developed. The frequent assay represents another choice to explicit individual assays to reduce the work associated to the prognosis of vitiviruses, along with for regulatory capabilities.

Pathologic full response (pCR) prices and outcomes after neoadjuvantchemoradiotherapy with proton or photon radiation for adenocarcinomas of the esophagus and gastroesophageal junction

Background: Pathologic full response (pCR) after neoadjuvantchemoradiotherapy (nCRT) is said to improved survival in victims dealt with for esophageal most cancers. Whereas proton beam treatment (PBT) has been demonstrated to reduce toxicities with nCRT, no info evaluating pCR prices between modalities exist to date. We investigated pCR prices in victims with distal esophageal/GEJ adenocarcinomas current course of trimodality treatment with nCRT-PBT or photon-based nCRT with the hypothesis that pathologic responses with PBT could be a minimal of as extreme as with photon treatment.

Methods: A single-institutional consider of victims with distal esophageal adenocarcinoma dealt with with trimodality treatment from 2015-2018 using PBT was achieved. PBT victims have been matched 1:2 to victims dealt with with photons. Chi sq. and two-sample t-tests have been utilized to examine traits, and the Kaplan Meier approach was used to estimate oncologic endpoints.

Outcomes: Eighteen consecutive PBT victims have been acknowledged and as compared with 36 photon victims. All victims obtained concurrent chemotherapy; 98% with carboplatin/paclitaxel. Most victims have been male (91%) and White (89%); median age was 62 years (fluctuate, 31-76 years). Median radiation dose in every cohorts was 50.4 Gy (fluctuate, 41.4-50.4 Gy); all applications have been delivered in 1.8Gy fractions. Age, gender and race have been successfully balanced.

Victims dealt with with PBT had a significantly larger pre-treatment nodal stage (N) and AJCC 7th model stage grouping (P=0.02, P=0.03). No matter this, tumoral and nodal clearance and pCR prices have been equal between cohorts (P=0.66, P=0.11, P=0.63, respectively). Complete pCR and explicit individual fundamental and nodal clearance prices, whole survival (OS), locoregional administration (LRC), and distant metastatic administration did not significantly differ between modalities (all P>0.05). Foremost perioperative events have been balanced; nonetheless, there have been 5 (14%) perioperative deaths inside the photon cohort as compared with 0 (0%) inside the proton cohort (P=0.06).

Conclusions: Utilizing PBT in trimodality treatment for distal esophageal adenocarcinoma yields pCR prices just like photon radiation and historic controls. Pathologic responses and oncologic outcomes on this analysis did not differ significantly between modalities no matter PBT victims having larger AJCC ranges and nodal sickness burdens.

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