A SYBR inexperienced I real-time polymerase chain response

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hydropsfetalis

A SYBR inexperienced I real-time polymerase chain response (PCR) assay for detection and quantification of Trichomonas gallinae

Trichomonas gallinae are parasitic flagellates of significance in wild and residential birds. The parasite is worldwide distributed, and Columbine birds are its foremost host. Current evaluation focuses completely on epidemiological and phylogenetic analysis. Nonetheless, there could also be nonetheless a lack of expertise regarding parasite-host interaction or treatment progress.

Precise-time PCR is a helpful gizmo for diagnostic and quantification of gene copies in a determined sample. By amplification of a 113-bp space of the 18S small subunit ribosomal RNA gene, a SYBR green-based real-time PCR assay was developed.

A daily curve was prepared for quantification analysis. Assay effectivity, linearity, and dissociation analysis have been effectively carried out. Specificity, sensibility, and reproducibility analysis have been examined. This assay is perhaps a helpful gizmo not only for diagnostic capabilities however moreover for future in vivo and in vitro T. gallinae analysis.

hydropsfetalis
hydropsfetalis

GPR12 Antibody

DF2721 100ul
EUR 280
Description: Human,Mouse,Rat

GPR12 Antibody

E11-323G 100μg
EUR 225
Description: Available in various conjugation types.

GPR12 Conjugated Antibody

C44910 100ul
EUR 476.4

GPR12 Polyclonal Antibody

ABP54586-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

GPR12 Polyclonal Antibody

ABP54586-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

GPR12 Polyclonal Antibody

ABP54586-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

GPR12 Polyclonal Antibody

ES5585-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA

GPR12 Polyclonal Antibody

ES5585-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA

GPR12 Polyclonal Antibody

E20-71963 100ug
EUR 225
Description: Available in various conjugation types.

GPR12 Antibody, HRP conjugated

1-CSB-PA009742LB01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

GPR12 Antibody, FITC conjugated

1-CSB-PA009742LC01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

GPR12 Antibody, Biotin conjugated

1-CSB-PA009742LD01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Polyclonal GPR12 Antibody (N-Terminus)

APR16481G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (N-Terminus). This antibody is tested and proven to work in the following applications:

Polyclonal GPR12 Antibody (Extracellular Domain)

APR16480G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (Extracellular Domain). This antibody is tested and proven to work in the following applications:

Polyclonal GPR12 Antibody - C-terminal region

APR16482G 0.05mg
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 - C-terminal region. This antibody is tested and proven to work in the following applications:

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx147559
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx324375
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx338465
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx009208
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

G Protein Coupled Receptor 12 (GPR12) Antibody (HRP)

20-abx335850
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

G Protein Coupled Receptor 12 (GPR12) Antibody (FITC)

20-abx335851
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

G Protein Coupled Receptor 12 (GPR12) Antibody (Biotin)

20-abx335852
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

GPR12

RA30050 50 ug
EUR 741.6

Gpr12/ Rat Gpr12 ELISA Kit

ELI-09764r 96 Tests
EUR 1063.2

GPR12 siRNA

20-abx918427
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

GPR12 siRNA

20-abx918428
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

GPR12 siRNA

20-abx902268
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

Anti-GPR12 antibody

STJ93328 200 µl
EUR 236.4
Description: Rabbit polyclonal to GPR12.

GPR12 Blocking Peptide

20-abx062679
  • EUR 326.40
  • EUR 493.20
  • 1 mg
  • 5 mg

GPR12 Blocking Peptide

DF2721-BP 1mg
EUR 234

GPR12 ELISA KIT|Human

EF005024 96 Tests
EUR 826.8

Rat GPR12 shRNA Plasmid

20-abx986567
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Human GPR12 shRNA Plasmid

20-abx951900
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Mouse GPR12 shRNA Plasmid

20-abx970621
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

anti- GPR105 antibody

FNab03588 100µg
EUR 658.5
Description: Antibody raised against GPR105

anti- GPR107 antibody

FNab03589 100µg
EUR 702
Description: Antibody raised against GPR107

anti- GPR108 antibody

FNab03590 100µg
EUR 658.5
Description: Antibody raised against GPR108

anti- GPR116 antibody

FNab03591 100µg
EUR 658.5
Description: Antibody raised against GPR116

anti- GPR132 antibody

FNab03593 100µg
EUR 606.3
Description: Antibody raised against GPR132

anti- GPR137 antibody

FNab03594 100µg
EUR 606.3
Description: Antibody raised against GPR137

anti- GPR142 antibody

FNab03597 100µg
EUR 702
Description: Antibody raised against GPR142

anti- GPR161 antibody

FNab03598 100µg
EUR 606.3
Description: Antibody raised against GPR161

anti- GPR162 antibody

FNab03599 100µg
EUR 702
Description: Antibody raised against GPR162

anti- GPR177 antibody

FNab03601 100µg
EUR 658.5
Description: Antibody raised against GPR177

GPR12 Recombinant Protein (Rat)

RP203285 100 ug Ask for price

GPR12 Recombinant Protein (Rat)

RP203288 100 ug Ask for price

GPR12 Recombinant Protein (Mouse)

RP139421 100 ug Ask for price

GPR12 Recombinant Protein (Mouse)

RP139424 100 ug Ask for price

GPR12 Recombinant Protein (Human)

RP061035 100 ug Ask for price

anti- GPR17 antibody

FNab03600 100µg
EUR 658.5
Description: Antibody raised against GPR17

anti- GPR22 antibody

FNab03603 100µg
EUR 606.3
Description: Antibody raised against GPR22

anti- GPR35 antibody

FNab03605 100µg
EUR 702
Description: Antibody raised against GPR35

anti- GPR50 antibody

FNab03607 100µg
EUR 658.5
Description: Antibody raised against GPR50

anti- GPR50 antibody

FNab03608 100µg
EUR 658.5
Description: Antibody raised against GPR50

anti- GPR81 antibody

FNab03610 100µg
EUR 702
Description: Antibody raised against GPR81

anti- GPR83 antibody

FNab03611 100µg
EUR 702
Description: Antibody raised against GPR83

anti- GPR137B antibody

FNab03595 100µg
EUR 702
Description: Antibody raised against GPR137B

Anti-GPR12/Gpcr12 Antibody

A10818 100ul
EUR 476.4
Description: Rabbit Polyclonal GPR12/Gpcr12 Antibody. Validated in IF and tested in Human.

Rabbit anti-GPR12 Antibody

DL90485A-100ul 100 ul
EUR 299
Description: G-protein coupled receptor 12

Rabbit anti-GPR12 Antibody

DL90485A-50ul 50 ul
EUR 209.3
Description: G-protein coupled receptor 12

Rabbit anti-GPR12 Antibody

YLD3545-100ul 100 ul
EUR 320
Description: Rabbit polyclonal antibody to GPR12

Rabbit anti-GPR12 Antibody

YLD3545-50ul 50 ul
EUR 200
Description: Rabbit polyclonal antibody to GPR12

anti- GPR3 antibody

FNab03604 100µg
EUR 702
Description: Antibody raised against GPR3

anti- GPR183/EBI2 antibody

FNab03602 100µg
EUR 658.5
Description: Antibody raised against GPR183/EBI2

Gpr12 ORF Vector (Rat) (pORF)

ORF067763 1.0 ug DNA
EUR 607.2

Gpr12 ORF Vector (Rat) (pORF)

ORF067764 1.0 ug DNA
EUR 607.2

GPR12 ORF Vector (Human) (pORF)

ORF020346 1.0 ug DNA
EUR 486

Gpr12 ORF Vector (Mouse) (pORF)

ORF046475 1.0 ug DNA
EUR 607.2

Gpr12 ORF Vector (Mouse) (pORF)

ORF046476 1.0 ug DNA
EUR 607.2

Anti-OR51E1/Gpr164 Antibody

A30874 100ul
EUR 476.4
Description: Rabbit Polyclonal OR51E1/Gpr164 Antibody. Validated in IF, WB and tested in Human.

Anti-LGR5/GPR49 Antibody

ER1902-52 100ul
EUR 189
Description: G protein-coupled receptors (GPCRs), also designated seven transmembrane (7TM) receptors or heptahelical receptors, interact with G proteins (heterotrimeric GTPases) to synthesize intracellular second messengers, such as diacylglycerol, cyclic AMP, inositol phosphates and calcium ions. Their diverse biological functions range from vision and olfaction to neuronal and endocrine signaling and are involved in many pathological conditions. LGR5 (leucine-rich repeat-containing G-protein coupled receptor 5), also known as GPR49 or GPR67, is a 907 amino acid multi-pass membrane protein that contains 17 leucine-rich repeats and belongs to the G protein-coupled receptor family. Expressed in placenta, skeletal muscle and spinal cord, LGR5 functions as an orphan receptor that is thought to play an important role in embryonic growth control and cellular differentiation. Overexpression of LGR5 is associated with increased tumor susceptibility and malignant transformation, implicating LGR5 as a potent tumor-inducing protein.

Anti-GPCR GPR40 Antibody

STJ501005 100 µg
EUR 571.2

Human GPR12 Protein Lysate 20ug

IHUGPR12PLLY20UG each
EUR 213
Description: Human GPR12 Protein Lysate 20ug

Anti-FFAR3/Gpr41 Antibody

A02437 100ul
EUR 476.4
Description: Rabbit Polyclonal FFAR3/Gpr41 Antibody. Validated in IF and tested in Human, Mouse.

anti- GPR101-Specific antibody

FNab03587 100µg
EUR 702
Description: Antibody raised against GPR101-Specific

anti- GPR123-Specific antibody

FNab03592 100µg
EUR 702
Description: Antibody raised against GPR123-Specific

anti- GPR139-Specific antibody

FNab03596 100µg
EUR 606.3
Description: Antibody raised against GPR139-Specific

Anti-SUCNR1/Gpr91 Antibody

A08485 100ul
EUR 476.4
Description: Rabbit Polyclonal SUCNR1/Gpr91 Antibody. Validated in WB and tested in Human, Mouse, Rat.

Anti-TAS1R2 / GPR71 antibody

STJ71396 100 µg
EUR 430.8

anti- GPR58/TAAR2 antibody

FNab03609 100µg
EUR 658.5
Description: Antibody raised against GPR58/TAAR2

Anti-GPCR GPR40 Antibody FITC

STJ501007 100 µg
EUR 703.2

anti- GPR37/Pael-R antibody

FNab03606 100µg
EUR 606.3
Description: Antibody raised against GPR37/Pael-R

Anti-GPCR GPR3 Antibody

STJ501253 100 µg
EUR 571.2

Anti-CXCR3 / GPR9 antibody

STJ70901 100 µg
EUR 430.8

Anti-GPCR GPR40 Antibody BIOTIN

STJ501006 100 µg
EUR 703.2

Anti-GPCR GPR40/FFAR1 Antibody

A01349-2 100ug/vial
EUR 352.8

Anti-LGR5/GPR49 Antibody [SU32-04]

ET1608-18 100ul
EUR 231
Description: Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5) also known as G-protein coupled receptor 49 (GPR49) or G-protein coupled receptor 67 (GPR67) is a protein that in humans is encoded by the LGR5 gene. It is a member of GPCR class A receptor proteins. R-spondin proteins are the biological ligands of LGR5. LGR5 is expressed across a diverse range of tissue such as in the muscle, placenta, spinal cord and brain and particularly as a biomarker of adult stem cells in certain tissues. LGR5 is a member of the Wnt signaling pathway. Although its ligand remains elusive, it has been shown that costimulation with R-spondin 1 and Wnt-3a induce increased internalization of LGR5. LGR5 also cointernalizes with LRP6 and FZD5 via a clathrin-dependent pathway to form a ternary complex upon Wnt ligand binding. Moreover, the rapid cointernalization of LRP6 by LGR5 induces faster rates of degradation for the former. LGR5 is crucial during embryogenesis as LGR null studies in mice incurred 100% neonatal mortality accompanied by several craniofacial distortions such as ankyloglossia and gastrointestinal dilation.

Gpr12 sgRNA CRISPR Lentivector set (Rat)

K7010601 3 x 1.0 ug
EUR 406.8

Anti-GPCR GPR3 Antibody FITC

STJ501255 100 µg
EUR 703.2

GPR12 sgRNA CRISPR Lentivector set (Human)

K0889201 3 x 1.0 ug
EUR 406.8

Gpr12 sgRNA CRISPR Lentivector set (Mouse)

K3382801 3 x 1.0 ug
EUR 406.8

Anti-GPCR GPR3 Antibody BIOTIN

STJ501254 100 µg
EUR 703.2

Anti-LGR4/Gpr48 Monoclonal Antibody

M02134 100uL
EUR 531.6
Description: Mouse Monoclonal LGR4/Gpr48 Antibody. Validated in IHC, WB and tested in Human.

Polyclonal Goat Anti-TAS1R2 / GPR71 Antibody

AMM05957G 0.1 mg
EUR 580.8
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-TAS1R2 / GPR71 . This antibody is tested and proven to work in the following applications:

Gpr12 3'UTR GFP Stable Cell Line

TU255326 1.0 ml Ask for price

Gpr12 3'UTR GFP Stable Cell Line

TU158969 1.0 ml Ask for price

GPR12 3'UTR GFP Stable Cell Line

TU059144 1.0 ml
EUR 1672.8

GPR12 Protein Vector (Rat) (pPM-C-HA)

PV271052 500 ng
EUR 723.6

GPR12 Protein Vector (Rat) (pPM-C-HA)

PV271056 500 ng
EUR 723.6

GPR12 Protein Vector (Rat) (pPB-C-His)

PV271050 500 ng
EUR 723.6

GPR12 Protein Vector (Rat) (pPB-N-His)

PV271051 500 ng
EUR 723.6

GPR12 Protein Vector (Rat) (pPM-C-His)

PV271053 500 ng
EUR 723.6

GPR12 Protein Vector (Rat) (pPB-C-His)

PV271054 500 ng
EUR 723.6

GPR12 Protein Vector (Rat) (pPB-N-His)

PV271055 500 ng
EUR 723.6

GPR12 Protein Vector (Rat) (pPM-C-His)

PV271057 500 ng
EUR 723.6

GPR12 Protein Vector (Mouse) (pPM-C-HA)

PV185900 500 ng
EUR 723.6

GPR12 Protein Vector (Mouse) (pPM-C-HA)

PV185904 500 ng
EUR 723.6

GPR12 Protein Vector (Human) (pPM-C-HA)

PV081383 500 ng
EUR 662.4

GPR12 Protein Vector (Mouse) (pPB-C-His)

PV185898 500 ng
EUR 723.6

GPR12 Protein Vector (Mouse) (pPB-N-His)

PV185899 500 ng
EUR 723.6

Enchancment of a typical RT-PCR assay for grapevine vitiviruses

The genus Vitivirus inside the family Betaflexiviridae comprises eleven viruses acknowledged to infect grapevine: grapevine vitiviruses A, B, D, E, F, G, H, I, J, L and M (GVA-GVM). Three of these viruses, GVA, GVB and GVD, have been associated to the etiology of rugose wood sickness in grapevine and set off agronomically important losses. The alternative vitiviruses have been additional simply recently discovered and their outcomes on grapevine are undetermined. To certify grape supplies for propagation as virus examined, an updated reverse transcription PCR (RT-PCR) assay to detect all acknowledged vitiviruses is fascinating.

To carry out this, various grapevine vitivirus sequences have been aligned on the amino acid diploma to hunt for conserved motifs. Two extraordinarily conserved motifs have been found at a superb distance for RT-PCR detection inside the RNA-dependent RNA polymerase space of the replicase protein. The amino acid motifs have been once more translated to create degenerate primers and used to effectively amplify all eleven grapevine vitiviruses. The RT-PCR primers have been used to examine a panel of vitivirus-infected vines for inclusivity along with vines contaminated with fastidiously related viruses inside the Betaflexiviridae family (i.e. grapevi)

ne pinot gris virus and grapevine rupestris stem pitting-associated virus) for exclusivity. Broader use of these primers to detect vitiviruses in numerous plant hosts was investigated. In summary, an end-point RT-PCR assay that detects all the acknowledged grapevine vitiviruses and doubtless totally different members of the genus Vitivirus has been developed. The frequent assay represents another choice to explicit individual assays to reduce the work associated to the prognosis of vitiviruses, along with for regulatory capabilities.

Pathologic full response (pCR) prices and outcomes after neoadjuvantchemoradiotherapy with proton or photon radiation for adenocarcinomas of the esophagus and gastroesophageal junction

Background: Pathologic full response (pCR) after neoadjuvantchemoradiotherapy (nCRT) is said to improved survival in victims dealt with for esophageal most cancers. Whereas proton beam treatment (PBT) has been demonstrated to reduce toxicities with nCRT, no info evaluating pCR prices between modalities exist to date. We investigated pCR prices in victims with distal esophageal/GEJ adenocarcinomas current course of trimodality treatment with nCRT-PBT or photon-based nCRT with the hypothesis that pathologic responses with PBT could be a minimal of as extreme as with photon treatment.

Methods: A single-institutional consider of victims with distal esophageal adenocarcinoma dealt with with trimodality treatment from 2015-2018 using PBT was achieved. PBT victims have been matched 1:2 to victims dealt with with photons. Chi sq. and two-sample t-tests have been utilized to examine traits, and the Kaplan Meier approach was used to estimate oncologic endpoints.

Outcomes: Eighteen consecutive PBT victims have been acknowledged and as compared with 36 photon victims. All victims obtained concurrent chemotherapy; 98% with carboplatin/paclitaxel. Most victims have been male (91%) and White (89%); median age was 62 years (fluctuate, 31-76 years). Median radiation dose in every cohorts was 50.4 Gy (fluctuate, 41.4-50.4 Gy); all applications have been delivered in 1.8Gy fractions. Age, gender and race have been successfully balanced.

Victims dealt with with PBT had a significantly larger pre-treatment nodal stage (N) and AJCC 7th model stage grouping (P=0.02, P=0.03). No matter this, tumoral and nodal clearance and pCR prices have been equal between cohorts (P=0.66, P=0.11, P=0.63, respectively). Complete pCR and explicit individual fundamental and nodal clearance prices, whole survival (OS), locoregional administration (LRC), and distant metastatic administration did not significantly differ between modalities (all P>0.05). Foremost perioperative events have been balanced; nonetheless, there have been 5 (14%) perioperative deaths inside the photon cohort as compared with 0 (0%) inside the proton cohort (P=0.06).

Conclusions: Utilizing PBT in trimodality treatment for distal esophageal adenocarcinoma yields pCR prices just like photon radiation and historic controls. Pathologic responses and oncologic outcomes on this analysis did not differ significantly between modalities no matter PBT victims having larger AJCC ranges and nodal sickness burdens.

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